Variable Sensitivity in Molecular Detection of Zika Virus in European Expert Laboratories: External Quality ... - Journal of Clinical Microbiology

1. Remi Charrel^a,
2. Ramona Mögling^b,
3. Suzan Pas^b,
4. Anna Papa^c,
5. Cecile Baronti^a,
6. Marion Koopmans^b,
7. Hervé Zeller^d,
8. Isabelle Leparc-Goffart^e and
9. Chantal B. Reusken^b

1. ^aUMR EPV Emergence des Pathologies Virales, Aix-Marseille Université, Marseille, France
2. ^bDepartment of Viroscience, Erasmus MC, Rotterdam, The Netherlands
3. ^cDepartment of Microbiology, Aristotle University of Thessaloniki, Thessaloniki, Greece
4. ^dEuropean Centre for Disease Prevention and Control (ECDC), Stockholm, Sweden
5. ^eFrench National Reference Centre for Arbovirus, Armed Forces Biomedical Research Institute, Marseille, France

ABSTRACT

Zika virus (ZIKV) infections are a significant public health concern. A strong capability for ZIKV detection is an absolute requirement for adequate preparedness and response strategies and individual patient care. The objective of this study was to assess and improve the capability of European expert laboratories for molecular testing for ZIKV through an external quality assessment (EQA) scheme. Laboratories were provided a panel of 12 samples which included negative samples, samples containing African- or Asian-lineage ZIKV at various concentrations (10³ to 10⁹ copies/ml), and samples containing dengue virus, yellow fever virus, or chikungunya virus. The results were analyzed on the basis of the outcomes of testing for the samples and the extraction and detection method used. Samples with a ZIKV RNA status scored correctly by >50% of the laboratories were designated the core sample. A total of 85 panel outcomes were submitted by 50 laboratories in 31 countries. The results designated all samples as core samples. Thirty-three percent (28/85) of the panel outcomes identified all samples. Analysis at the laboratory level showed that only 40% of the laboratories (20/50), representing 45% of the countries, scored sufficiently; i.e., they had at least one test operational that scored all core samples correctly. There is a need for improvement of the molecular detection of ZIKV in 60% of the participating laboratories. While the specificity of the tests was more robust, the results of the EQA showed large variation in test sensitivity. Improvements should focus on both nucleic acid extraction and ZIKV detection methods.

KEYWORDS

FOOTNOTES

• • Received 10 July 2017.
  • Returned for modification 26 July 2017.
  • Accepted 21 August 2017.
  • Accepted manuscript posted online 23 August 2017.
• Address correspondence to Chantal B. Reusken, c.reusken{at}erasmusmc.nl.

• R.C. and R.M. contributed equally to this article.

• Citation Charrel R, Mögling R, Pas S, Papa A, Baronti C, Koopmans M, Zeller H, Leparc-Goffart I, Reusken CB. 2017. Variable sensitivity in molecular detection of Zika virus in European expert laboratories: external quality assessment, November 2016. J Clin Microbiol 55:3219–3226. http://ift.tt/2gBgsFO.

• Supplemental material for this article may be found at http://ift.tt/2gBgsFO.

• Copyright © 2017 American Society for Microbiology.

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